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. 2017 Mar 8;13(5):3118–3126. doi: 10.3892/ol.2017.5826

Figure 1.

Figure 1.

miR-195 expression is downregulated and is inversely correlated with AEG-1 expression in HCC. (A) RT-qPCR was used to measure the expression levels of miR-195 in eight human liver cancer cell lines (HepG2, Huh7, PLC/PRF/5, SMMC-7721, SK-HEP-1, MHCC-97H, MHCC-97L and Hep 3B) and a normal human hepatic cell line (L02). U6 was used as an internal control and the mean miR-195 expression level in L02 liver cells was designated as 1. (B) The expression of miR-195 was detected in 36 paired HCC and tumor-adjacent NT samples by RT-qPCR. miR-195 was downregulated in HCC tissues compared with the adjacent NTs. The central horizontal line represents the mean value. (C) AEG-1 mRNA was detected in 36 paired HCC and NT samples by RT-qPCR. β-actin was used as internal control and the central horizontal line represents the mean value. (D) miR-195 expression is inversely correlated with AEG-1 expression (R2=0.308; P<0.01, Pearson's correlation). *P<0.05. miR-195, microRNA-195; AEG-1, astrocyte elevated gene 1; HCC, hepatocellular carcinoma; RT-qPCR, reverse transcription-quantitative polymerase chain reaction; NT, normal tissue.