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. 2017 Mar 8;13(5):3118–3126. doi: 10.3892/ol.2017.5826

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Copyright: © Yan et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 4. — Rescue of miR-195 ectopic expression effects by simultaneous overexpression of AEG-1. (A) Expression of AEG-1 protein in Huh7 cells. Proteins were extracted and analyzed by western blot at 48 h after co-transfection with miR-NC or miR-195 mimic and pcDNA3.1-AEG-1 or pcDNA3.1 in Huh7 cells. GAPDH was used as an internal control. (B) Cell proliferation detected in Huh7 cells at 0, 24, 48 and 72 h after transfection. (C) Cell apoptosis detected by Annexin-V/propidium iodide combined labeling flow cytometry in Huh7 cells at 48 h after transfection. Apoptotic evaluation was determined by the percentage of the apoptotic cell number out of the total cell number. miR-195, microRNA-195; AEG-1, astrocyte elevated gene 1; miR-NC, negative control microRNA.