Figure 5.

Overexpression of miR-195 impairs the growth of hepatoma xenografts in vivo. (A) Relative expression of miR-195 in Huh7 cells transfected with lentivirus LV-miR-195 or LV-miR-NC was analyzed by reverse transcription-quantitative polymerase chain reaction. The average miRNA expression in LV-miR-NC group was designated as 1. *P<0.05. (B) Fluorescence microscopy was applied to detect the transfection efficiency of the lentivirus in Huh7 cells (right panels). The images in the left panels show the cells in the same field under normal white light. (C) Dynamic growth curves of xenografted tumors. Huh7 cells transfected with LV-miR-NC or LV-miR-195 were injected subcutaneously into nude mice. From the 10th day after injection, tumor sizes were measured every 4 days and tumor growth curves were obtained. Data are presented as the mean tumor volume ± standard error (n=6). (D) Mice were sacrificed and representative images of tumor volumes were captured at 30 days after injection. miR-195, microRNA-195; AEG-1, astrocyte elevated gene 1; miR-NC, negative control microRNA.