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. 2017 May 8;7:1558. doi: 10.1038/s41598-017-01318-x

Table 1.

List of quantitative primers used.

Primers name Primer sequence (5′-3′) Tm value (°C) Amplified fragment length (bp)
Qtubulin YF AGACAGAATCAGAAAGTTGGCAGA 63.2 220
Qtubulin YR GTGAGTGGTCAGGATGGAGTTG 63.9
QHIF YF TATTTGATGGGCTGTTCTGCTC 62.0 280
QHIF YR AGTAATGGGGTGCCAACTGCTA 65.1
QALDO YF ACCACAAACGAAACGACAACAC 63.6 219
QALDO YR AGGCTCCACAATAGGCACCA 65.0
QPDK YF GCCTTGATAGCGGTAGTCCATA 62.7 200
QPDK YR CGATTTGCCAAGAGTTGAAGTG 61.6
QPHD YF TGGTATTACGAGATTTGATGTGTTG 60.5 253
QPHD YR TTCATCTTGCTTGCTGATACTTTGT 62.4
QLDH YF AGAAAACTGCTGCTGGATACAACT 63.8 289
QLDH YF TGAAATTCAAGAAATAACGATGGAG 59.0