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. 2017 May 8;7:1532. doi: 10.1038/s41598-017-01695-3

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Relative cellular miR155-5p expression is higher in M1 stimulation phenotypes. To examine the effects of monocyte/macrophage stimulation, we compared relative cellular miR155-5p expression levels in the various monocyte/macrophage groups to monocytes cultured without stimulant (A: CD14 vs. No stimulant; B: LPS vs. No stimulant; C: IFNɣ vs. No stimulant; D: IL4 vs. No stimulant; E: TGFβ1 vs. No stimulant). Data presented are based on quantitative polymerase chain reaction measurements of miR155-5p in monocyte/macrophages of the total patient cohort (n = 26) with high (CFI_p > 0.39) or low (CFI_p < 0.39) collateral capacity. Data are presented as mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001. CFI_p: Collateral flow index; LPS: Lipopolysaccharide; IFNɣ: Interferon gamma; IL4: Interleukin 4; TGFβ1: Transforming growth factor beta 1.