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. 2017 May 8;7:1567. doi: 10.1038/s41598-017-01703-6

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Indels are susceptible to variant calling edge effects as shown by simulation. (A) Simulation scheme of 420 insertions and 420 deletions with 20 different lengths at 21 different positions. (B) Venn diagram of called insertions or deletions in 3 approaches of primer handling. (C) Heat map of length and position of called insertions or deletions.