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. 2017 May 8;7:1542. doi: 10.1038/s41598-017-01606-6

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Comparison of localization performance by theoretical calculation and numerical simulation. (a) Comparison of relative localization precision for the objectives with different NAs. (b) Comparison of localization precision for different cameras under different signal levels. (c) Comparison of localization precision between the low-cost SMLM and the standard SMLM under different signal levels using simulated images. 1000 images were simulated for each data point. Here, the standard SMLM uses TIRF objective (100X, NA1.49) and EMCCD camera with a pixel size of 160 nm.