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. 2017 May 8;7:1531. doi: 10.1038/s41598-017-01723-2

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effects of lentivirus transduction and doxycycline-induced ChR2 expression on NRVM cultures. (A and B) Fluorescent and phase-contrast images, respectively, of a ChR2-transduced monolayer; (C) Phase-contrast image of a control monolayer; (D) Confocal image of ChR2-expressing cardiomyocytes; (E and F) Fluorescent and phase-contrast images, respectively, of a ChR2-expressing monolayer pretreated with BrdU; (G) ChR2 transduction efficiency in cell cultures with or without BrdU pretreatment (two-tailed unpaired Student’s t test, n = 6); and (H) Effect of BrdU on ChR2-eYFP fluorescence intensity averaged over monolayer (two-tailed unpaired Student’s t test, n = 6).