Figure 2.

Dexamethasone stimulates phosphorylation of CaMKII and Syn in mouse pituitary explants. Explanted pituitaries were treated with Dex (10⁻⁷ M) or not (Vehicle, V) for 30 min. Lysates were subjected to immunoblotting with anti-p-CaMKII, anti-CaMKII, anti-Syn, anti-p-Syn and anti-α-tubulin antibodies. Dex induced significant increase in the phosphorylation state of CaMKII and Synapsin-I. Cropped blots (full length WBs are presented in Supplementary Fig. S4d) are shown in the figure and are representative image of 3 independent experiments. Results are expressed as means ± SEM fold stimulation when compared to vehicle. Statistical significance was assessed by One way ANOVA, Dunnett’s posttest, n = 3, *p ≤ 0.05. p-CaMKII: phospho-CaMKII; p-Syn: phospho-synaspin-I.