Figure 4.
GC prevent GnRH-induced CaMKII phosphorylation. LβT2 cells were treated or not (V) with GnRH (10−8 M), Cort (10−7 M), Cort-BSA (10−7 M) or co-treated with either GnRH and Cort or GnRH and Cort-BSA for 5 min. Cell lysates were subjected to WB with anti-CaMKII, anti-p-CaMKII, and anti-α-tubulin antibodies. Cropped blots (full length WBs are presented in Supplementary Fig. S4e) are shown in the figure and are representative of 3 independent experiments. Results are expressed as means ± SEM fold stimulation when compared to vehicle. One-way ANOVA with Dunnett’s post-test, **p < 0.01. ## p < 0.01 comparing GnRH with both co-treatments.