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. 2017 May 8;7:1548. doi: 10.1038/s41598-017-01528-3

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Stem cell marker expression of spheroids. (A) Immunohistology for nuclear stem cell markers, NANOG, OCT4 and SOX2, in ASCs after 12 h culture on 2D petri dish, non-adhesive dish, and HA gel. Nuclei were labeled with Hoechst (blue). Scale bars = 50 µm. (B) SSEA-3 expression in ASCs 12 h after seeding on 2D petri dish (2D), non-adhesive dish (NAD), and in HA gel (HA). Bar graph showed the relative quantification of the SSEA-3 fluorescence. p = 0.019, *^,**p < 0.05; n = 3 independent experiments. Scale bars = 100 µm.