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. 2017 May 8;7:1548. doi: 10.1038/s41598-017-01528-3

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Immunohistological tracing of administered ASCs in irradiated skin ulcers of nude mice. (A) Immunohistological views of irradiated ulcer specimens 13 days after surgery and transplantation for tracing of administered ASCs (DiI-labeled cells). Cell fates were visualized by immunostaining for perilipin, Mac-2, and αSMA. Scale bars = 50 µm. (B) Whole mount staining of the irradiated wound tissue at days 7 and 13. Vascular endothelial cells were labeled by isolectin. Scale bars = 50 µm.