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. 2017 Mar 22;13(5):3508–3516. doi: 10.3892/ol.2017.5893

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Copyright: © Wang et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 5. — RP5-899B16.2 silencing affects the viability of gallbladder carcinoma cell lines. Expression of RP5-899B16.2 in (A) GBC-SD and (B) SGC-996 cell lines transfected with control siRNA or two siRNAs targeting RP5-899B16.2 (siRNA-1 and siRNA-2). 18S was used as the internal control. MTT assay of (C) GBC-SD and (D) SGC-996 cells transfected with NC, siRNA-1 and siRNA-2. Relative cell viability with respect to NC-treated cells (set as 1) of the (E) GBC-SD and (F) SGC-996 cell lines 96 h following siRNA transfection. siRNA-1: siRP5-899B16.2-1; siRNA-2: siRP5-899B16.2-2; *P<0.05; ***P<0.001. siRNA, small interfering RNA; NC, negative control; OD, optical density.