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. 2017 May 8;7:1547. doi: 10.1038/s41598-017-01686-4

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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¹HNMR spectra of PVP and NPVP. The peaks at 8.25 and 6.72 ppm were ascribed to the protons at the 2, 6 sites and 3, 5 sites of pyridine ring. After N-alkylation, they shifted to lower magnetic field of 7.68 and 8.91 ppm. Signal at 4.53 ppm was corresponding to the protons of the methylene groups bonded with N atom in the pyridine ring. The peaks from 1.31 to 1.86 ppm came from the aliphatic protons from the PVP backbone, the protons of methylene groups from alkyl bromides. The protons of methyl groups from alkyl bromides were observed in the high field of 0.88 ppm.