Table 1.

Calculation of the Relative Transcript Abundance using the ΔΔCt Method

ActD Treatment (min)	Ct (Fos)	Ct (Actb)	Mean Ct (Actb)	ΔCt*	Mean ΔCt	Δ ΔCt**	2−ΔΔCt	Mean	SD
0	27.2821	18.0651	18.0561	9.2260	9.2308	−0.0047	1.0033	1.0000	0.0046
0	27.2915	18.0470		9.2355		0.0047	0.9967
10	28.5733	18.0886	18.0314	10.5419		1.3112	0.4030	0.4183	0.0217
10	28.4676	17.9741		10.4362		1.2054	0.4336
20	30.2251	17.7979	17.7688	12.4563		3.2255	0.1069	0.1057	0.0018
20	30.2593	17.7397		12.4905		3.2598	0.1044
30	32.1141	18.0410	18.0095	14.1046		4.8739	0.0341	0.0295	0.0065
30	32.5635	17.9780		14.5540		5.3232	0.0250
45	34.6656	17.9821	18.0185	16.6471		7.4163	0.0059	0.0050	0.0012
45	35.1741	18.0549		17.1555		7.9248	0.0041
60	35.9865	18.2261	18.1696	17.8169		8.5862	0.0026	0.0026	0.0000
60	35.9577	18.1131		17.7881		8.5573	0.0027
90	38.0750	18.0576	17.9927	20.0823		10.8515	0.0005	0.0007	0.0002
90	37.5847	17.9279		19.5919		10.3612	0.0008
120	36.7232	17.8345	17.8792	18.8440		9.6133	0.0013	0.0009	0.0005
120	38.0775	17.9240		20.1983		10.9676	0.0005
240	38.0583	18.0231	17.9790	20.0792		10.8485	0.0005	0.0010	0.0006
240	36.7326	17.9350		18.7536		9.5228	0.0014

^*ΔCt = Ct (Fos, column 2) − Ave Ct (Actb, column 4);

^**ΔΔCt = ΔCt (column 5)−Ave ΔCt at time 0 (column 6).

Actinomycin D (ActD) was added after 30 min of TNF stimulation.