Figure 4.

JTT does not enhance tumor vaccine efficacy. Female BALB/c mice were fed the control or JTT diet for three weeks and then challenged s.c. with 1×10⁶ live syngeneic CT26 cells. These mice continued to be fed the same diets until four weeks following the tumor challenge. (A) Tumors were measured twice weekly. Each experiment comprised four to six mice per group. These experiments were repeated at least twice with similar results. The results are presented as the mean ± SD. (B) Irradiated (20,000 rad) CT26 cells (1×10⁵) were injected s.c. into BALB/c mice. Vaccinated mice were fed the control diet or JTT diet from the time of the vaccination until four weeks following the tumor challenge. Three weeks following the vaccination, 1×10⁶ live CT26 cells were s.c. injected into these mice and tumors were then measured twice weekly. Each experiment included five to six mice per group. Results were pooled from two independent experiments. The results are presented as the mean + SD. P<0.01, Mann-Whitney test between the control diet group and vaccine plus control diet group on day 28 following the CT26 challenge; P<0.02, Mann-Whitney test between the control diet group and vaccine plus JTT diet group on day 28 following the CT26 challenge. s.c., subcutaneously; JTT, juzentaihoto; SD, standard deviation.