Fig. 5.
Mice treated with lysed L. reuteri have more oxytocin-immunoreactive (OT-ir) neurons in the caudal PVN. (A) Representative photomicrographs depicting oxytocin-ir neurons in the caudal PVN of mice treated with normal drinking water (Untreated N = 9), or live L. reuteri (N = 8), or lysed L. reuteri (N = 10). The numerical value in the bottom right corner of top plates represents the distance (in millimeters) posterior to bregma for the rostral, intermediate and caudal PVN. (B) Average number of oxytocin-ir cells observed in the rostral, intermediate, and caudal PVN of mice treated with normal drinking water (Untreated), live L. reuteri, or lysed L. reuteri. *p < 0.05 (Tukey post hoc tests following one-way ANOVA).