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. 2017 Feb 5;7(3):e2126. doi: 10.21769/BioProtoc.2126

Table 1. Preparation of sucrose stock solutions.

Sucrose Gradient %	10%	20%	30%	40%	50%
2.2 M Sucrose	24 ml	48 ml	72 ml	96 ml	120 ml
H₂O	138 ml	114 ml	90 ml	66 ml	42 ml
10X salts solution	18 ml	18 ml	18 ml	18 ml	18 ml
TOTAL volume	180 ml	180 ml	180 ml	180 ml	180 ml