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. 2017 Mar 28;13(5):3727–3733. doi: 10.3892/ol.2017.5931

Figure 4.

Figure 4.

CALB1 depletion causes necrosis and apoptosis of osteosarcoma U2OS cells. (A) The apoptotic death of U2OS cells administered with three treatments, Con-shRNA, CALB1-shRNA 1 and CALB1-shRNA 2, was determined through Annexin V-APC/7-AAD double staining and analyzed using flow cytometry. (B) Deficiency of CALB1 in U2OS cells could trigger necrosis, and early and late apoptosis. APC−/7-AAD− and APC−/7-AAD+ along the horizontal axis represent viable and necrotic cells, while APC+/7-AAD− and APC+/7-AAD+ represent early and late apoptotic cells respectively. *P<0.05, **P<0.01, and ***P<0.001 vs. Con-shRNA. CALB1, calbindin 1; sh, short hairpin; con, negative control.