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RT, forward and reverse primers for RT-quantitative polymerase chain reaction analysis of miR-638 and U6.
| Primer | Sequence |
|---|---|
| miR-638 | |
| RT | 5′-GTCGTATCCAGTGCGTGTCGTGGAGTCG |
| GCAATTGCACTGGATACGACAGGCCGC-3′ | |
| Forward | 5′-ATCCAGTGCGTG TCGTG-3′ |
| Reverse | 5′-TGCTAGGGATCGC GGGCGGGTG-3′ |
| U6 | |
| RT | 5′-CGCTTCACGAATTTGCGTGTCAT-3′ |
| Forward | 5′-GCTTCGGCAGCACATATACTAAAAT-3′ |
| Reverse | 5′-CGCTTCACGAATTTGCGTGTCAT-3′ |
RT, reverse transcription; miR, miRNA.
