Table I.
Primary antibodies and conditions used for immunohistochemistry.
| Antibody | Cat. no. | Clone | Company | Species/clonality | Dilution, incubation duration and temperature |
|---|---|---|---|---|---|
| E-cadherin | 999 | 42E | Cell Signalinga | Rabbit/MC | 1:200, 1 h at RT |
| Vimentin | 413541 | SP20 | Nichireib | Rabbit/MC | 1:200, 1 h at RT |
| Snail-1/Snail-2 | ab63371 | N/A | Abcamc | Rabbit/PC | 1:200, O/N at 4°C |
| p-P38 MAPK | 4631 | 12F8 | Cell Signalinga | Rabbit/MC | 1:50, O/N at 4°C |
| NANOG | 79923 | D73G4 | Cell Signalinga | Rabbit/MC | 1:800 O/N at 4°C |
a
Cell Signaling Technology, Inc., Danvers, MA, USA
b
Nichirei Biosciences, Inc., Tokyo, Japan
c
Abcam, Cambridge, UK. MC, monoclonal; PC, polyclonal; RT, room temperature; O/N, overnight; p-p38 MAPK, phosphorylated p38 mitogen activated protein kinase; Snail; zinc finger protein SNAI; NANOG, homeobox protein NANOG.