Figure 4.

PsA induced apoptosis in HT-29 cells. (A) HT-29 cells were treated with a vehicle control or PsA, 5-FU, 5-FU+PsA for 3 h. The cells were incubated with an anti-annexin-V antibody and analyzed by flow cytometry. (B) The percentage of Annexin V positive cells was calculated. The data presented in the bar graph were obtained from three independent experiments. (C) HT-29 cells were treated for 6–8 h. Percentage of apoptotic tumor cells were observed by fluorescence microscopy. Magnification, ×40. PsA, Pulsatilla saponin A; 5-FU, fluorouracil; Con, control.