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A) Purity of the macrophage culture was determined to be ~90% by immunofluorescent quantification of F4/80 and CD11b, murine macrophage markers B) The deficiency of Mac-1 receptor on macrophages from the Mac-1 KO mouse was verified by staining for CD11b (α chain of Mac-1). The percentage of cells expressing CD11b was determined to be ~1%.
