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. 2017 May 10;7:1646. doi: 10.1038/s41598-017-01602-w

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(A) Total serum bilirubin is not decreased in 4 weeks old male Gunn rats after bi-daily intraperitoneal injection of 150 mg/kg Montelukast for 2, 5 days (5 injections), compared to animals receiving vehicle control. Data represent the mean ± SD of three animals per group. (B) Concentration of Montelukast in serum, liver, spleen and kidney of 4 weeks old male Gunn rats after bi-daily intraperitoneal injection of 150 mg/kg Montelukast for 2, 5 days (5 injections). Animals were sacrificed 5 hours after last Montelukast administration. Tissue homogenates were made in 100 mmol/L Tris-HCL pH 7, 8 buffer and concentration of Montelukast was measured by HPLC. Data represent the mean ± SD of three animals per group.