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. 2017 May 11;7:1732. doi: 10.1038/s41598-017-01814-0

Figure 4.

Figure 4

Insulin secretion in MIN6 and islets is modulated by KCC2. (A) Insulin secretion in MIN6 β-cells under basal (5.5 mM glucose) or stimulated conditions (7.5–20 mM glucose), either in the presence of ML077 (25 µM) or CLP257 (100 µM). Results are expressed as percentage of total insulin content and normalized to Control 5.5 mM ± SEM (n = 3, *p < 0.05). (B) Dose-response curve of the basal secretory response (5.5 mM glucose) of MIN6 β-cells as a function of increasing concentrations of ML077 (1–100 µM). Shown are the results of 2 different experiments performed in triplicate. (C) Insulin secretion of mouse islets and MIN6 incubated in low or high glucose (3.3–16.6 mM for islets or 5.5–15 mM for MIN6, respectively). Results of insulin secretion are expressed relative to total insulin content (n = 3, *p < 0.05 vs. glucose alone).