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. 2017 May 11;7:1778. doi: 10.1038/s41598-017-01914-x

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Schematic representation of the in vivo laser assisted bioprinting geometries tested, namely a ring (A1) with external and internal diameter of 3 and 2.1 mm, respectively, and a disk (B1) with 2 mm diameter. In both cases, two layers of nHA-collagen ink were printed underneath and over the cellularized ink layer. Representative fluorescence images of ring (A2) and disk (B2) printed tomato-positive (D1) cells inside the calvaria defect in mice, immediately after printing.