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. 2017 May 11;7:1778. doi: 10.1038/s41598-017-01914-x

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(A) Representative X-ray micro tomography (μCT) reconstruction images of nHA collagen and D1 cells printed in a ring or disk geometry (calvaria defect in the right side), or nHA collagen alone (calvaria defect in the left side), at 2 months post printing in a mice calvaria model. Horizontal (B) and coronal (C) μCT projection and regions of interest (3.3 mm diameter and 0.5 mm thick disk) for the evaluation of bone repair, in a calvaria defect in mice at 2 months post impression. (D) Quantitative assessment of bone volume/total volume (BV/TV) by μCT evaluation of nHA collagen and D1 cells, printed in a ring or disk geometry, or nHA collagen alone at 1 and 2 months post printing (Average ± SD, n = 9, *and ***denote p < 0.05 and p < 0.001, respectively).