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. 2017 May 11;7:1759. doi: 10.1038/s41598-017-01956-1

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Schematic of colorimetric single-cell pH imaging and detection with two pH indicators. (a) Schematic of single-cell pH imaging and detection by combined use of bright-field microscope-based UV-Vis microspectroscopy and common pH indicators. A selected area single-cell absorption spectrum is collected by an optical microscope equipped with a portable spectrometer and an optical splitter with a small collecting area. (b) The structures of two pH indicators used in this study.