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. 2017 May 11;7:1759. doi: 10.1038/s41598-017-01956-1

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Visual identification and screening of cancer cells from normal cells and cytotoxicity assays. (a) Bright-field optical microscope images of cancerous HepG2, HeLa, A549, 4T1, normal HL7702 and L929 cells before and after 5 min incubation with 1 mg mL⁻¹ of bromothymol blue, respectively; All scale bars: 20 μm. (b) Cytotoxicity assays for the A549, HeLa, HL7702, and L929 cells after the incubation with bromothymol blue or bromocresol green. A, B, C, D under the X-coordinate represent A549, HeLa, HL7702, and L929 cells, respectively. (c) Bright-field microscopy images of cocultured cancerous 4T1 and normal HL7702 cells before (left, scale bar: 50 μm) and after (right, scale bar: 15 μm) the treatment with bromocresol green. The cells within the white dotted line are identified as HL7702 cell.