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. 2017 May 11;7:1773. doi: 10.1038/s41598-017-01808-y

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Comparison of metabarcoding data with traditional monitoring data on zooplankton species monitoring. (A) Frequency of occurrence of each species by metabarcoding and morphological method. (B) The top panel shows the proportion of OTUs in metabarcoding data matched to the morphological identification at each site. Red color indicates the proportion of species detected by both morphological method and metabarcoding. Grey color indicates the proportion of species detected by metabarcoding only. The bottom panel shows the proportion of species detected by metabarcoding at each sample. (e.g. 0.8 means 80% morphological species detected by metabarcoding).