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. 2017 May 11;7:1748. doi: 10.1038/s41598-017-01576-9

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Diet and FXR deficiency changed gut microbiota composition in both genders. (A) Cecal microbiota at phylum level. (B) Firmicutes to Bacteroidetes ratio. Principal component analysis plots of cecal microbiota at family level based on diet (C), phenotype (D), and gender difference (E). (F) and (G), relative abundance of cecal microbiota at family level (Kruskal-Wallis test). Box plots display the median, 25th percentile, and 75th percentile; whiskers display minimum and maximum values. (H) Targeted functional quantitative PCR analysis of microbial genes. (B,H), data are expressed as mean ± SD. One-way ANOVA with Tukey’s correction. n = 16 in male groups, n = 6 in female groups. *p < 0.05, **p < 0.01, ***p < 0.001 for diet comparison; ^# p < 0.05, ^## p < 0.01, ^### p < 0.001 for genotype comparison; ^& p < 0.05, ^&& p < 0.01, ^&&& p < 0.001 for gender comparison.