Figure 2.
NRG1 treatment does not affect the main regulators of energy homeostasis in gastrocnemius muscle. C57BL/6JRJ (C57) control and db/db (Db) male mice were treated with vehicle (VHL; 0.9% NaCl solution; n = 8/each condition) or with NRG1 (50 μg . kg−1; n = 8/each condition), three days per week for eight weeks. Western blot analysis ((A) cropped images) was used to quantify the abundance of AMPK (B), ACC (D), ACL (F) and their phosphorylation ratios (C,E and G) relative to the level in untreated healthy mice (C57-VHL, white bars). Results are the mean ± SEM (n = 8 per group). The diabetes (healthy vs db/db mice) and NRG1 (saline vs NRG1) effects were investigated with a 2 × 2 ANOVA. When a significant interaction was found, the Tuckey’s test was used for post-hoc multiple comparisons. **p < 0.01, NS: not significant.