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. 2017 May 11;7:1801. doi: 10.1038/s41598-017-01985-w

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Variation in RBC aggregation measured by analyzing image intensity. (a) Temporal variation in flow rate of blood samples estimated by micro-PIV technique. (b) A series of speckle images for blood samples after the lapse of 1, 3, and 6 h from LPS injection with respect to measurement time. The size of each image is 128 × 128 pixels, corresponding to 0.64 × 0.64 mm² in the physical dimension. (c) Temporal variations in the normalized light intensity (I/I ₀) of blood samples after 1, 3, and 6 h from LPS injection at the center of the channel. (d) Comparison of aggregation index (α) according to the measurement time after LPS injection. Light intensities of blood samples at 6 h after LPS injection, and the corresponding fitting curves are inserted. (^*p < 0.005 and ^**p < 0.001: significant difference between groups).