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. 2017 May 10;7:1713. doi: 10.1038/s41598-017-01974-z

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(a) Comparison of chill coma recovery dynamics in CA (blue squares) vs. CO (red circles) flies. Time to recover from chill coma was monitored in flies recovering at 25 °C after 9 h of chronic cold stress at 0 °C (n = 40). (b) Climbing activity monitored in CA vs. CO flies recovering flies after 2, 4 and 6 h following 9 h at 0 °C. Flies were categorized as fit (white part) or recovering (grey part) or injured (black part) (n = 50). (c) Critical thermal minimum (CT_min) in the two groups tested. The horizontal lines indicate the mean value (n = 21). Mortality rate (black part) in CA and CO flies recovering for 24 h at 25 °C after 9 h of chronic cold stress at 0 °C (n = 50) (d) or 2 h of acute cold stress at −3.5 °C (n = 50) (e). Symbols (*) indicate a significant difference (p < 0.05).