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. 2017 May 10;7:1713. doi: 10.1038/s41598-017-01974-z

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Volcano plot constructed from weighed spectral counts. The plot shows how much and how significant the identified proteins were differentially abundant between CA and CO flies. Horizontal dotted line depicts a p < 0.05 cutoff and vertical dotted lines depict 2- and 0.5-fold cutoffs. Among 64 significant differential proteins (i.e. above cutoff values), 18 were identified without phosphorylated peptides (grey squares), 12 were phosphorylated and less abundant in the CA samples (red circles) and 34 were phosphorylated and more abundant in the CA samples (blue circles).