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. 2017 May 12;7:1880. doi: 10.1038/s41598-017-01971-2

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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A role for Celsr1 in planar cell division orientation across the mammalian epidermis. (A) polar plots of total planar oriented cell divisions in E16 Celsr1 Crsh mutant litters. E15.5 litters exhibited a similar pattern to E16 (data not shown). Blind analyses were performed for wild-type and heterozygote Crsh littermates, >4 embryos from 4 litters for each group and for each wild-type. (B,D) histograms of PCD and long axis orientation, range of bin widths is shown along the X-axis. (B,D’) scatter plots of PCD and long axis orientation, mean and SD are shown. (C) whisker box plots showing the extent of the longest axis (length between the two shortest opposing interfaces) of basal epithelial cells for each condition. Box plots show maximum and minimum, median and 75% and 25% percentile values. Statistical analysis was Student’s t-test, *denotes P-value > 0.01; **denotes P-value > 0.001; ***denotes P-value > 0.0001.