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. 2017 May 12;7:1854. doi: 10.1038/s41598-017-02019-1

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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H7N9 virus-specific T cells responses. Two and 4 weeks after immunization, mouse PBMCs (peripheral blood mononuclear cells) were separated and collected. PBMCs were stimulated with H7HA peptide pools (10 μg/mL) 2 hours before adding GolgiPlug^TM for another 4 hours of incubation. The percentage of CD8⁺ T cells secreting IFN-γ was then measured by intracellular cytokine staining. (a) H7N9 virus-specific T cell responses at 2 weeks after prime immunization. (b) H7N9 virus-specific T cell responses at 4 weeks after prime immunization. The error bars represent the SD. ***p < 0.001; **p < 0.01; *p < 0.05. ^#At 2 weeks post immunization, the prime-boost group received DNA priming, as in the DNA-only group.