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. 2017 May 12;7:1854. doi: 10.1038/s41598-017-02019-1

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Antibody responses in guinea pigs. Twelve female guinea pigs were divided into 4 groups: the AdC68-H7HA group, the pCAGGS-H7HA group, the AdC68-gp group, and the prime-boost group. Guinea pigs were administered doses double those in mice. Serum samples were collected every 2 weeks for antibody detection. (a) Total IgG in guinea pigs taken at different times after immunization. (b) HAI titres in guinea pigs at 4, 8, and 12 weeks after immunization. (c) NT titres in guinea pigs at 4, 8, and 12 weeks after immunization. ***p < 0.001; **p < 0.01; *p < 0.05. ^#At two weeks post immunization, the prime-boost group received DNA priming, as in the DNA-only group.