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. 2017 May 10;7:1689. doi: 10.1038/s41598-017-01584-9

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Prior material testing, the differentiation of monocytes to M2-like macrophages was confirmed. (A) Macrophage markers CD14, CD68, and CD206 were expressed. (B) The differentiation towards a M2-like phenotype was shown by CD163 positive profile, whereas (C) CD80 allowed distinguishing between M1 and M2. Here, its absence rejected the M1 differentiation. The marker CD197 was expressed by macrophages differentiated from whole-blood-derived monocytes. Histograms exemplarily represent the differentiation cluster of one donor; whereas mean values ± SD represent data of three blood donors. A high compliance between all three donors was found. The light-grey histograms represent the isotype controls. The following abbreviations are used: SD for standard deviation, CD for cluster of differentiation.