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. 2017 May 10;7:1689. doi: 10.1038/s41598-017-01584-9

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Chemokine IL-8, and anti-inflammatory cytokine IL-10 revealed significant differences between the test conditions, whereas pro-fibrotic growth factor TGF-β1 did not resolve differences. (A) Here, we demonstrated a test condition dependency of IL-8 levels for tests on glass. (B) Additionally, IL-10 levels differed significantly between test conditions on glass, PTFE and silicone surfaces. (C) Macrophages’ secretion of TGF-β1 did not exceed the basal level found in the culture medium, thereby no effect of the test conditions were resolved. Those cytokines were analyzed in the supernatant of ten macrophage donors (n = 10). A p-value ≤ 0.05 is considered as significant. LPS-induced secretion of cytokines is provided in separate graphs (see Figure S1). The following abbreviations are used: WO for without treatment, HI for heat-inactivated.