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. 2017 May 10;7:1684. doi: 10.1038/s41598-017-01943-6

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Proliferation assay of Raji cells expressing f3 and f9 or after treatment with JQ1. (a) Non-induced (gray) and induced cells (25 ng/ml doxycycline, green) were plotted. Induced cells expressed either luciferase (Raji-luc, negative control) or dominant-negative Brd4 mutants f3 and f9. (b) Percentage of induced cells (green) were assessed by measuring GFP reporter signals using flow cytometry. Uninduced Raji f2 cells served as negative control (gray). (c) Raji cells were treated with increasing concentrations of JQ1 for 72 hours. Cell proliferation was measured via MTS assay and plotted against JQ1 concentration (log scale). (d) Induced Raji-luc cells were treated with 500 nM JQ1 (red)/DMSO (gray, control) for 8 days. Living cell numbers were counted and plotted against time.