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. 2017 May 12;7:1850. doi: 10.1038/s41598-017-01833-x

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(a) Diffraction amplitude from a specimen with a mitochondrion together with Au reference objects. The central part is enlarged in an inset to display details of the speckle pattern. (b) CXDI projection image of the mitochondrion reconstructed from (a). The red circular objects are the Au reference objects. An image with an enlarged field of view is included in the upper right corner. (c) PRTF evaluated from the iterative reconstruction which indicates that the image resolution is around 14 nm. Red dotted line indicates 1/e, the resolution criterion. (d–f) AFM (d), fluorescence (e), and SEM (f) image of the same specimen. The green fluorescence emission indicated in (e) shows that the measured object is indeed a mitochondrion. The scale bars are 400 nm.