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. 2017 May 11;7:1724. doi: 10.1038/s41598-017-01850-w

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Identification of putative 3-HP-responsive genes in P. putida KT2440 and C. necator H16. (a) Operons putatively involved in 3-HP metabolism in P. putida KT2440 and C. necator H16. They are composed of the TR coding sequences hpdR, mmsR or araC, and divergently transcribed genes which are putatively required for 3-HP degradation: hpdH, 3-hydroxypropionate dehydrogenase; mmsA, methylmalonate-semialdehyde dehydrogenase; mmsB, 3-hydroxyisobutyrate dehydrogenase and acaD, acyl-CoA dehydrogenase. (b) Schematic illustration of the modular reporter system. It contains the four unique restriction sites AscI, FseI, PmeI and SbfI which are used for modular assembly. The application-specific module harbours the rfp reporter gene and the switchable system composed of transcriptional regulator and inducible promoter. (c) The P. putida KT2440 hpdR/hpdH intergenic region. Translational start sites are bold and italicised. The predicted hpdH −35 and −10 regions are bold and highlighted in grey. The nucleotide sequence between position −118 and −68 relative to the P. putida KT2440 hpdH translational start site is underlined. (d) A sequence similarity motif was generated which corresponds to the underlined sequence. It represents highly conserved nucleotides in the hpdR/hpdH intergenic regions of forty analysed Pseudomonas species.