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. 2017 May 11;7:1724. doi: 10.1038/s41598-017-01850-w

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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P. putida KT2440 hpdH promoter truncations. (a) Schematic illustration of the different hpdH promoter truncations that were evaluated for 3-HP-responsive activation of rfp reporter gene expression. The native promoter-reporter gene construct is denoted hpdH-233::rfp. The bioinformatically identified palindromic sequence is illustrated as a dashed box. This sequence was replaced by an AvrII restriction site, CCTAGG, resulting in promoter truncations hpdH-118::rfp and hpdH-106::rfp. (b) Induction levels mediated by the different promoter truncations. The various promoter-reporter gene constructs were analysed in E. coli MG1655 for RFP expression in the absence or presence of 10 mM 3-HP. Error bars represent standard deviations of three biological replicates.