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. 2017 May 11;7:1724. doi: 10.1038/s41598-017-01850-w

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Induction dynamics and kinetics of the PpHpdR/P_hpdH switchable system. (a) Relative fluorescence and absorbance curves of E. coli MG1655 and C. necator H16 cultures harbouring the native PpHpdR/P_hpdH switchable system, pEH008. 3-HP was supplemented at time zero to the final concentrations of 10, 5, 2.5, 1.25, 0.625, 0.156, 0.016 mM and no inducer. The darker the shade of colour, the higher the level of 3-HP. The standard error of three biological replicates is illustrated as band. (b) Dose response curve of the PpHpdR/P_hpdH switchable system in E. coli MG1655 and C. necator H16. It illustrates the relation between inducer concentration and fluorescence output 4 (circle), 6 (square) and 8 (triangle) hours after 3-HP addition. Inducer levels range from 0, 0.016, 0.078, 0.156, 0.313, 0.625, 1.25, 2.5, 5 and 10 mM. Error bars represent standard deviations of three biological replicates.