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. 2017 May 10;7:1669. doi: 10.1038/s41598-017-01804-2

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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DNA segmentation of HeLa (A) and CaSki (B) cells detected by DAPI stain. TUNEL-assay for detection of apoptosis in HeLa (C) and CaSki (D) cells. (E,F) Apoptosis of HeLa cells detected by the Annexin V-FITC/PI staining test and the ratio of apoptosis cells. The data represented the means ± SD for triplicate determinations. *P < 0.05, **P < 0.01 versus control cells. (G,H) Apoptosis of CaSki cells detected by the Annexin V-FITC/PI staining test and the ratio of apoptosis cells, including early apoptotic cells (lower right quadrant) and late apoptotic cells (upper right quadrant). The data represented the means ± SD for triplicate determinations. *P < 0.05, **P < 0.01 versus control cells.