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. 2017 May 10;7:1669. doi: 10.1038/s41598-017-01804-2

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Zey induces apoptosis of HeLa and CaSki cells through caspase activation. (A) Zey induced expression changes of FAS, FADD, Bid, pro-caspase 8, and cleaved-caspase 8. (B) Zey induces release of AIF and cytochrome C from mitochondria and cleavage of caspase 9. HeLa and CaSki cells were treated with Zey for 24 h, then cell cytoplasm were extracted by digitonin buffer and the released AIF and cytochrome C were detected by western blot analysis. (C) Zey induces cleavage of PARP, caspases-3, and -7 in HeLa and CaSki cells. (D) Zey decreased expression of antiapoptotic proteins Bcl-XL, and Bcl-2, and increased expression of apoptotic proteins Bax and Bad. Zey inhibits caspase 3 activity of HeLa (E) and CaSki cells (F). Data were presented as the means ± SD for triplicate determinations. *P < 0.05, **P < 0.01 versus control cells. Pan caspase inhibitor Z-VAD-FMK significantly attenuated Zey-induced apoptosis in HeLa (G) and CaSki cells (H). Data are presented as the means ± SD for triplicate determinations. **P < 0.01 versus control cells.