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. 2017 May 11;7:1754. doi: 10.1038/s41598-017-01918-7

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Molecular characterization of TaPIMP2-overexpression wheat plants and responses to Bipolaris sorokiniana. (a) Scheme of the TaPIMP2 expression cassette on the transformation vector pUBI::myc-TaPIMP2. Ubi: maize ubiquitin promoter; Tnos: terminator of Agrobacterium tumefaciens nopaline synthase gene. The arrow indicates the amplified regions of transgenic plants by PCR. (b) PCR patterns of the TaPIMP2-transgenic wheat lines using the primers specific to the TaPIMP2-Tnos cassette. M: molecular marker; P: plasmid pUBI::myc-TaPIMP2; Y16: the recipient Yangmai 16. (c) Relative expression levels of TaPIMP2 in overexpressing wheat lines and untransformed Yangmai 16. The relative transcript level in the untransformed Yangmai 16 was set to 1. (d) Western blotting analysis of the TaPIMP2-overexpressing wheat lines and untransformed Yangmai 16 using an anti-myc antibody. (e) The typical common root rot symptoms of TaPIMP2-overexpression and untransformed Yangmai 16. Values represent the mean and standard deviation from three independent biological replicates (Student’s t-test: *P < 0.05).