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. 2017 May 10;7:1698. doi: 10.1038/s41598-017-01730-3

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Increased pEAT levels of CEL in HFDMG and GK rats (A), calculated as percentage of Ct, while no differences were observed for GLO-1; representative WB are shown. Glycation increases glycoconjugates, fibrosis and CEL staining in pEAT. Histological analysis shows PAS (100X) (B), Masson Trichrome (100X) (C) and CEL staining (100X) (D). Ct - Wistar 12 m; MG - Wistar + MG supplementation; HFD - HF diet-fed Wistar; HFDMG - HF diet-fed Wistar + MG supplementation; GK - Goto-Kakizaki 12 m. Bars represent means ± SEM, n = 6–8. * vs Ct; ^$ vs HFD. 1 symbol p < 0.05; 2 symbols p < 0.01.