Fig. 3.

GI is required in vivo for complete ZTL activity. a Relative-specific activity of ZTL-LUC at ZT1 and ZT13 in the Col and gi-201 background. b Relative-specific activity of LUC from the CCR2:LUC transgene in the Col and gi-2 backgrounds. c Relative-specific activity of ZTL^WT-, ZTL^G46E-, and ZTL^C82A-LUC at ZT1 and ZT13 in the Col background. a–c Specific activity was determined by the ratio of luminescence (enzyme activity) to LUC protein levels derived from ZTL^WT-, ZTL^G46E-, ZTL^C82A-LUC or LUC protein alone. See “Methods” for details. Data are means ± s.e. of eight (a) or four (b, c) independent samples